The combination of artificial intelligence (AI) and advanced microscopy is radically changing the research in life sciences. Machine learning and deep learning can deliver unparalleled image-processing tasks, such as automated classification, super-resolution reconstruction, and real-time counts of biological structures. This synergy promotes the discovery process in crucial fields like drug discovery, systems biology and clinical diagnostics by increasing the speed, accuracy and reproducibility of data analysis. This review is a synthesis of the present state of this integration, critical analysis of its transformative capacity and ongoing issues. The most important developments are mentioned, including the automated phenotyping and predictive modeling, to the AI-enhanced super-resolution imaging, and its effect on precision medicine and high-throughput screening. Nonetheless, there are still considerable challenges, such as the problem of data standardization, model interpretability, and incorporating multimodal imaging data. The future direction of the field is described, including the new solutions, which are explainable AI (XAI), federated learning as a privacy-preserving collaboration, and integrated bioinformatics pipelines. AI-powered microscopy is a paradigm shift, a new way to view and ask questions of microscopic images, bridging microscopic visualization to computational intelligence