Molecular Profiling of Multidrug-Resistant Escherichia coli Harboring tetA and eaeV3 Genes in Poultry Production System

Authors

  • Hosen Md. Aoulad Hajee Mohammad Danesh Science and Technology University, Dinajpur -5200,Bangladesh Author
  • Mahe Afroz Department of Microbiology, Facuulty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author
  • Nazmi Ara Rumi Department of Microbiology, Facuulty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author
  • Md. Aoulad Hosen Department of Microbiology, Facuulty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author
  • Md. Shajedur Rahman Department of Medicine, Surgery and Obstetrics, Facuulty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author
  • Most. Deloara Begum Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author
  • Md. Shiblee Sadik Sabuj Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author
  • Nasrin Sultana Tonu Upazila Livestock Office and Veterinary Hospital, Barura, Cumilla Author
  • Md. Hasanul Baker Tamlikha Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh Author

DOI:

https://doi.org/10.71005/xfdn0c79

Keywords:

Serogrouping , Bangladesh, Poultry, Microbial, Resistance

Abstract

Escherichia coli, a common gastrointestinal pathogen in warm-blooded animals, poses a significant challenge topoultry health. This study investigated the prevalence, serogroup distribution, and molecular characteristics of multidrug-resistant (MDR) E. coli in dead chickens from Dinajpur, Bangladesh. A total of 131 organ samples (intestine, liver, heart, lungs, oviduct) were analyzed using cultural, biochemical (Indole, MR-VP, TSI, citrate), and molecular (PCR) methods. E. coli was detected in 41.22% (n=54)of samples, with the highest prevalence in intestines (90%) from Basherhat (p=0.021). Molecular identification of the isolates was confirmed by 16S rRNA gene sequencing (585 bp). The resistance genes TetA and EAEV3 were detected in 29.6% and 14.8% of isolates, respectively. Serogroup D was the most prevalent (61.11%). All E. coli showing 100% resistance to several classes, including penicillins, cephalosporins, macrolides, and tetracyclines. In contrast, they remained 100% susceptible to fluoroquinolones (ciprofloxacin, norfloxacin) and aminoglycosides (gentamicin, streptomycin). Among the isolates, 34 (68.51%) were classified as MDR. The findings underscore the escalating threat of antimicrobial resistance (AMR), which poses significant challenges to poultry health management. The results also raise public health concerns due to the potential transmission of resistant E. coli through the consumption of contaminated poultry products. Therefore, implementing measures such as restricting antibiotic misuse, strengthening surveillance, and improving farm biosecurity is critical to combat the AMR crisis in this poultry sector.

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Published

2025-12-30

Issue

Vol. 2025 No. 1 (2025): Special Issue

Section

Original research

How to Cite

[1]
Md. Aoulad, H. et al. 2025. Molecular Profiling of Multidrug-Resistant Escherichia coli Harboring tetA and eaeV3 Genes in Poultry Production System. Atlantic Journal of Life Sciences. 2025, 1 (Dec. 2025). DOI:https://doi.org/10.71005/xfdn0c79.